anti p enos  (Cell Signaling Technology Inc)

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: Both p-AKT/AKT and eNOS expression are downregulated in dental pulp from diabetic patients. (A and B) Immunohistochemistry staining results of eNOS in dental pulp tissue in NT group and DM group. (C-E) Western Blot confirmed the phosphorylation degree of AKT and eNOS in NT group and DM group. Scale bar: 100 μm. Data presented as mean ± SD.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, Phospho-proteomics

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: High glucose affects the proliferation and differentiation of hDPSCs and inhibits the expression of eNOS in hDPSCs via the AKT pathway. (A) Analysis of CCK-8 assay in DPSCs cultured in media with different glucose concentrations. (B) Quantification of ALP activity in DPSCs cultured in media with different glucose concentrations. (C) Quantification of ALP activity in DPSCs from blank, mannitol and HG groups. (D-F) Western Blot confirmed the proteins level of p-AKT, AKT and eNOS in blank, HG and SC79 group. Data are presented as mean ± SD.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Expressing, CCK-8 Assay, Cell Culture, Activity Assay, Western Blot

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: Knockdown of eNOS inhibits the osteo/odontogenic differentiation capacity of hDPSCs. (A and B) ALP staining and quantification of ALP activity results in vector and eNOSsh group. (C and D) Alizarin red staining and Ca 2+ ion measurement results in vector and eNOSsh group. (E-G) Western Blot confirmed the proteins level of DSPP and DMP1 in vector and eNOSsh group. (H-K) RT-qPCR results showed the downregulation of RUNX2(H), OCN(I), BSP(J), and DSPP(K) mRNA expression levels in eNOSsh group. Data are presented as mean ± SD.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Knockdown, Staining, Activity Assay, Plasmid Preparation, Western Blot, Quantitative RT-PCR, Expressing

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: Overexpression of eNOS could rescue the impairment of osteo/odontogenic capability of hDPSCs in high-glucose conditions. (A and B) ALP staining and quantification of ALP activity results. (C and D) Alizarin red staining and Ca 2+ ion measurement results. (E-G) Western Blot confirmed the proteins level of DSPP and DMP1. (H-K) RT-qPCR results showed the upregulation of mRNA expression levels of BSP(H), RUNX2(I), DSPP(J), and DMP1(K) in eNOS group. Data are expressed as mean ± SD.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Over Expression, Staining, Activity Assay, Western Blot, Quantitative RT-PCR, Expressing

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: Overexpression of eNOS in DPSCs promotes dentin regeneration in the extraction sockets of rabbit jaws. (A and B) Micro-CT analysis. (C) Observation of cross-section in the lower 1/3 segment of the extraction socket. (D) Gross observation of regeneration tissue in rabbit extraction socket. Data presented as mean ± SD.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Over Expression, Extraction, Micro-CT

Journal: International Dental Journal

Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis

doi: 10.1016/j.identj.2026.109511

Figure Lengend Snippet: Histological examination of regenerated tissues in the extraction sockets of rabbit jaws. (A and B) HE staining results. (C and D) SEM examination of regeneration tissues in rabbit extraction socket. (E-G) Immunohistochemistry staining results of DSPP in regenerated dentin-like tissue in the vector group and eNOS group. (H-J) Immunohistochemistry staining results of DMP1 in regenerated dentin-like tissue in the vector group and eNOS group. Scale bar: 200 μm (A), 100 μm (B, E, H), 50 μm (F, I), 5 μm (C), 2 μm (D). Data presented as mean ± SD. AB: alveolar bone; rD: regenerated dentin-like tissue; Od and black arrow: odontoblast-like cells.

Article Snippet: Short hairpin RNA (shRNA) lentiviral vectors for NOS3 knockdown, NOS3 overexpression vectors, and control lentiviral vectors were constructed by GeneChem.

Techniques: Extraction, Staining, Immunohistochemistry, Plasmid Preparation